Yes there is good evidence that acetaminophen (the active ingredient in Tylenol) can deplete glutathione under certain conditions. But whether that matters depends on dose, duration, and individual factors. g glutamyl cycle. Depletion of glutathione by acetaminophen leads to Download Scientific Diagram Xia & He Publishing Does Acetaminophen Deplete Glutathione? Understanding the Connection Cymbiotika CYMBIOTIKA Paracetamol toxicity Biochemical Basis Lecture Oxidative stress during acetaminophen hepatotoxicity: Sources, pathophysiological role and therapeutic potential ScienceDirect